What question did this study set out to answer?

To determine whether prostaglandins synthesized by Cox-1 or Cox-2 regulate crypt epithelial cell apoptosis, prostaglandin synthesis, and stem cell survival following radiation-induced injury.

November 1, 2000AJP Gastrointestinal and Liver Physiology

Disruption of cyclooxygenase-1 gene results in an impaired response to radiation injury

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Authors

CHCourtney W. HouchenOklahoma State University Oklahoma City WSWilliam F. StensonBoston University SCSteven M. CohnThe University of Texas Medical Branch at Galveston

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Implication

Animal study reveals impaired crypt stem cell survival following radiation in Cox-1-deficient mice, indicating that Cox-1-derived prostaglandins protect the intestinal epithelium.

Key Points

To determine whether prostaglandins synthesized by Cox-1 or Cox-2 regulate crypt epithelial cell apoptosis, prostaglandin synthesis, and stem cell survival following radiation-induced injury.
Subjected Cox-1(-/-), Cox-2(-/-), and wild-type control mice to gamma-irradiation.
Assessed intestinal crypt epithelial apoptosis, clonogenic stem cell survival, and PGE2 synthesis, including the effect of a specific PGE2 neutralizing antibody.
Cox-1(-/-) mice demonstrated increased crypt epithelial cell apoptosis, decreased clonogenic stem cell survival, and diminished baseline and post-injury PGE2 synthesis compared with wild-type littermates.
Cox-2(-/-) mice exhibited levels of apoptosis, stem cell survival, and intestinal PGE2 synthesis after irradiation that did not differ from wild-type controls.
Inhibition of crypt stem cell survival post-irradiation by a neutralizing antibody to PGE2 confirmed that PGE2 mediates stem cell fate in vivo.

Cite This Study

Houchen et al. (2000) studied this question.

synapsesocial.com/papers/6a92ade29da65ccb784a18af https://doi.org/10.1152/ajpgi.2000.279.5.g858

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Implication

Animal study reveals impaired crypt stem cell survival following radiation in Cox-1-deficient mice, indicating that Cox-1-derived prostaglandins protect the intestinal epithelium.

Disruption of cyclooxygenase-1 gene results in an impaired response to radiation injury

Discussion

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Implication

Key Points

Cite This Study

Disruption of cyclooxygenase-1 gene results in an impaired response to radiation injury

Discussion

Member takes

Implication

Key Points

Cite This Study

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