The Changes of Heart miR-1 and miR-133 Expressions following Physiological Hypertrophy Due to Endurance Training.

Figures

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  Fig.1. The weight trend (means) in the rats during 14 weeks of endurance training. W; Week.

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  Fig.2. Two-dimensional targeted M-mode echocardiograms from the heart of one rat from the endurance-trained (ET) group. RVAWT; Right ventricular anterior wall thickness, RVD; Right ventricular dimension, ST; Septum thickness, LVDd; Left ventricular diameter diastole, LVDs; Left ventricular diameter diastole, and PWT; Posterior wall thickness.

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  Fig.3. Expressions of miR-1 and miR-133 in the rats. Representative image of real-time polymerase chain reaction (PCR) results for miR-1 and miR- 133 in left ventricle tissue from the control (CON) and experimental groups (ET), respectively. The expressions of the microRNAs (miRNAs) were normalized to U6 expression. Histogram of real-time PCR miRNA products showed that both miR-1 and miR-133 had significantly increased expressions of greater than~2.1-fold (P=0.001 and P=0.004, respectively) in response to 14 weeks of endurance training). Values are mean ± SE (n=7). Data were analysed by the independent t test.∗∗; P<0.01.

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  Fig.4. Expressions of histone deacetylase 4 (Hdac4) and heart and neural crest derivatives express 2 (Hand2) genes in rats. Representative image of real-time polymerase chain reaction (PCR) results for Hdac4 and Hand2 in left ventricle tissue from the control (CON) and experimental groups (ET), respectively. Expressions of the genes were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression. Histogram of real-time PCR gene products revealed significantly increased expression of both genes by ~143-fold (P=0.001) in response to 14 weeks of endurance training. Values are mean ± SE (n=7). Data were analysed by the independent t test.∗∗; P<0.01.

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  Fig.5. Expressions of Mef2c and Srf genes in the rats. Representative image of real-time polymerase chain reaction (PCR) results for Mef2c and Srf genes in left ventricle tissue from the control (CON) and experimental groups (ET), respectively. The gene expressions were normalized to glyceraldehyde- 3-phosphate dehydrogenase (GAPDH) expression. Histogram of real-time PCR gene products revealed that Srf expression was significantly increased by ∼5.9-fold (P=0.001) in response to 14 weeks of endurance training, whereas there was no difference in the expression level of Mef2c between the CON and ET groups (P=0.148). Values are mean ± SE (n=7). Data were analysed by the independent t test. **; P<0.01.