Key result
Injecting engineered anti-inflammatory macrophages into high fat diet-fed obese mice triggered white adipose tissue browning, reduced pro-inflammatory responses, and improved insulin sensitivity.
Why the study?
Does injecting engineered anti-inflammatory macrophages improve diet-induced insulin resistance and induce white adipose tissue browning in high-fat diet-fed obese mice?
Does injecting engineered anti-inflammatory macrophages improve diet-induced insulin resistance and induce white adipose tissue browning in high-fat diet-fed obese mice?
Delivering engineered anti-inflammatory macrophages triggers white fat browning, stimulates whole-body thermogenesis, and reduces obesity-associated insulin resistance in a mouse model.
Should not change practice; hypothesis-generating for engineered macrophage therapy in obesity-related metabolic disease.
We recently exploited a transgenic approach to coerce macrophage anti-inflammatory M2 polarization in vivo by lowering Receptor Interacting Protein 140 (RIP140) level in macrophages (mφRIP140KD), which induced browning of white adipose tissue (WAT). In vitro, conditioned medium from cultured adipose tissue macrophages (ATMs) of mφRIP140KD mice could trigger preadipocytes' differentiation into beige cells. Here we describe a cell therapy for treating high fat diet (HFD)-induced insulin resistance (IR). Injecting M2 ATMs retrieved from the WAT of mφRIP140KD mice into HFD-fed obese adult wild-type mice effectively triggers their WAT browning, reduces their pro-inflammatory responses, and improves their insulin sensitivity. These data provide a proof-of-concept that delivering engineered anti-inflammatory macrophages can trigger white fat browning, stimulate whole-body thermogenesis, and reduce obesity-associated IR.
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Liu et al. (2014) studied High fat diet (HFD)-induced insulin resistance. Engineered anti-inflammatory macrophages (M2 ATMs from mφRIP140KD mice) was evaluated on White adipose tissue browning, pro-inflammatory responses, and insulin sensitivity. Injecting engineered anti-inflammatory macrophages into high fat diet-fed obese mice triggered white adipose tissue browning, reduced pro-inflammatory responses, and improved insulin sensitivity.
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