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June 1, 2019The CRISPR JournalOpen Access

Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13

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Authors

OAOmar O. AbudayyehJGJonathan S. GootenbergMKMax J. Kellner

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Overview

Experimental study demonstrates rapid detection of herbicide resistance genes in crude soybean extracts, highlighting portable field-ready diagnostics for crop breeding.

Key Points

  • To adapt the CRISPR-Cas13-based SHERLOCK platform for rapid, portable detection and quantification of specific plant genes from crude agricultural samples.
  • Engineered a modified CRISPR-Cas13 SHERLOCK assay targeting glyphosate resistance genes and multiplex plant targets.
  • Evaluated platform functionality directly on crude soybean extracts with minimal sample preparation using a color-based lateral flow readout.
  • SHERLOCK enabled target plant gene detection and quantitation within approximately 15 minutes.
  • The system successfully identified genes directly from crude soybean extracts without requiring complex nucleic acid purification.
  • A color-based lateral flow strip enabled visual multiplex detection of multiple plant targets within a single reaction.

Cite This Study

Abudayyeh et al. (2019) studied this question.

synapsesocial.com/papers/6a92bc2271c7499eda183d0bhttps://doi.org/10.1089/crispr.2019.0011
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