Solvent Effects in Reversed Phase Liquid Chromatooraphy for High and Low Loadings of Octadecylsilane on Microparticle Silica. I

The selectivity of two ODS bonded phase packings, one with a high carbon load, the other with a low carbon load, were investigated. Many organic solvent/water mobile phases were used with three sample mixtures of varying polarity. When the separations were normalized, trends in the solubility parameters of the various classes of organic solvents were noted. Analyzing the separation factors and considering possible mobile phase, solute, and bonded phase interactions furnished some insight into possible separation/selectivity mechanisms.