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A triacontyl (C30) stationary phase has been synthesized on a silica hydride surface for use in preparative applications. The success of bonding via hydrosilation was monitored by diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy and elemental (carbon) analysis. A semi-preparative column was packed in order to test this material for potential applications. The test compounds selected for this evaluation were carotenoids. A number of organic solvents applicable to either normal or reversed-phase phase retention were used as binary mobile phase components. The effect of flow rate on resolution was also determined.
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Pesek et al. (2011) studied this question.
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