What question did this study set out to answer?

To synthesize a novel calcium alginate hydrogel formulation and determine its immunomodulatory effects on human monocyte and macrophage function.

February 20, 2025International Journal of Biological MacromoleculesOpen Access

A novel calcium alginate hydrogel formulation to enhance monocyte/macrophage anti-inflammatory activity

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Authors

NHNell HirtInserm MAM. AlkobtawiInserm EMEnzo ManchonInserm

Discussion

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Implication

In vitro study demonstrates calcium alginate hydrogel induces anti-inflammatory phenotypes in human monocytes and macrophages, indicating therapeutic promise for inflammatory disorders.

Key Points

To synthesize a novel calcium alginate hydrogel formulation and determine its immunomodulatory effects on human monocyte and macrophage function.
Formulated a novel calcium-alginate hydrogel and co-cultured it with monocyte subsets and polarized M1 macrophages.
Evaluated surface marker expression (CD36, CD64, CD206, CD163), TNFα production, mitochondrial mass, and reactive oxygen species generation.
Assessed immunomodulatory activity using circulating monocytes collected from patients with recessive dystrophic epidermolysis bullosa.
Hydrogel exposure significantly downregulated inflammatory markers CD36 and CD64, upregulated CD206, and reduced TNFα secretion across monocyte subsets.
M1 macrophages shifted toward an anti-inflammatory CD163+CD206+ profile, accompanied by increased mitochondrial mass and suppressed reactive oxygen species generation.
In monocytes from patients with recessive dystrophic epidermolysis bullosa, the hydrogel expanded classical subsets, diminished intermediate inflammatory subsets, and suppressed TNFα, CD36, and CD64.

Cite This Study

Hirt et al. (2025) studied this question.

synapsesocial.com/papers/6a92c3166a4f73282e1849fe https://doi.org/10.1016/j.ijbiomac.2025.141251

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February 20, 2025International Journal of Biological MacromoleculesOpen Access

A novel calcium alginate hydrogel formulation to enhance monocyte/macrophage anti-inflammatory activity

View Full Paper

Ask AI

Bookmark

Authors

NHNell HirtInserm MAM. AlkobtawiInserm EMEnzo ManchonInserm

Discussion

Member takes

Implication

In vitro study demonstrates calcium alginate hydrogel induces anti-inflammatory phenotypes in human monocytes and macrophages, indicating therapeutic promise for inflammatory disorders.

Key Points

To synthesize a novel calcium alginate hydrogel formulation and determine its immunomodulatory effects on human monocyte and macrophage function.
Formulated a novel calcium-alginate hydrogel and co-cultured it with monocyte subsets and polarized M1 macrophages.
Evaluated surface marker expression (CD36, CD64, CD206, CD163), TNFα production, mitochondrial mass, and reactive oxygen species generation.
Assessed immunomodulatory activity using circulating monocytes collected from patients with recessive dystrophic epidermolysis bullosa.
Hydrogel exposure significantly downregulated inflammatory markers CD36 and CD64, upregulated CD206, and reduced TNFα secretion across monocyte subsets.
M1 macrophages shifted toward an anti-inflammatory CD163+CD206+ profile, accompanied by increased mitochondrial mass and suppressed reactive oxygen species generation.
In monocytes from patients with recessive dystrophic epidermolysis bullosa, the hydrogel expanded classical subsets, diminished intermediate inflammatory subsets, and suppressed TNFα, CD36, and CD64.

Cite This Study

Hirt et al. (2025) studied this question.

synapsesocial.com/papers/6a92c3166a4f73282e1849fe https://doi.org/10.1016/j.ijbiomac.2025.141251

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