Effects of rate of cooling to 5°C, storage at 5°C, glycerol concentration, sodium chloride, fructose and glycine on the revival of deep-frozen bull spermatozoa

Bull spermatozoa were deep-frozen to −79° C. in diluents prepared with 1% lecithin and containing mixtures of sodium citrate, sodium chloride, fructose, glycine and gelatin, and revival rates measured after thawing, using scores of motility rate of progression of spermatozoa), the visual estimation of percentage of motile spermatozoa and the percentage of spermatozoa unstained by Congo-red-nigrosin. The experimental design was a one-ninth replicate of a 37 factorial. A decrease in the time taken to cool samples