The concentration of HMf (histone Methanothermus fervidus) in vivo has been shown to be between 1 and 2 × 104 molecules per genome. At this mass ratio the amount of HMf bound to pUC19 DNA in vitro was found to be dependent on the topology of the plasmid DNA. M. fervidus grows optimally between 80 and 85°C and contains approx. 1 M K+ plus 300 mM 2′3′(cyclic) diphosphoglycerate. (Hensel, R. and König, H. 1988. FEMS Microbiol. Lett. 49, 75–79). HMf binding to DNA in vitro under these conditions has been demonstrated.
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Stroup et al. (1992) studied this question.
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