Key result
A prior contractile period significantly reduced the time delay before intracellular PO2 began to decrease at the onset of contractions in single skeletal muscle fibers (5 vs 13 seconds, P<0.01).
Why the study?
Does a prior contractile period alter the kinetics of intracellular PO2 fall at the onset of contractions in Xenopus single skeletal muscle fibers?
Population
Intact single skeletal muscle fibers (n = 11) from Xenopus laevis
Comparison
A protocol consisting sequentially of 1-min… vs First contractile period vs. second contractile…
Design
Preclinical
Authors
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Prior contraction hastens PO2 on-kinetics in isolated fibers; hypothesis-generating for intracellular control of oxidative phosphorylation, no clinical translation yet.
Does a prior contractile period alter the kinetics of intracellular PO2 fall at the onset of contractions in Xenopus single skeletal muscle fibers?
Absolute Event Rate: 5% vs 13%
p-value: p=<0.01
In Xenopus skeletal muscle fibers, a prior contractile period results in more rapid on-kinetics of oxidative phosphorylation, suggesting intracellular factors other than O2 availability determine these kinetics.
Michael C. Hogan (2001) studied this question. Prior contractile work (second contractile period) vs. Rested state (first contractile period) was evaluated on Time delay before intracellular PO2 began to decrease after the onset of contractions (p=<0.01). A prior contractile period significantly reduced the time delay before intracellular PO2 began to decrease at the onset of contractions in single skeletal muscle fibers (5 vs 13 seconds, P<0.01).
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