Peroxidase‐catalyzed electrochemical assay of hydrogen peroxide: A ping–pong mechanism

In view of the great importance of determination of hydrogen peroxide in many scientific fields and industrial applications and the attractive operational simplicity of potentiometric approach for the enzymatic assay, the kinetics of horseradish peroxidase–catalyzed electrochemical assay of H2O2 was studied in this work at 25°C. All kinetic characteristics were determined by the double reciprocal Lineweaver–Burk and (primary and secondary) double reciprocal Hanes–Woolf plots. The results confirmed that the reaction follows a ping–pong mechanism. The Michaelis–Menten constants for H2O2 and 4‐fluorophenol were Km = 0.081 ± 0.001 mM and K4‐FPm = 0.185 ± 0.002 mM, respectively. The maximum rate was also estimated to be Vmax = 0.182 ± 0.002 mM min−1 (at 25 ± 0.05°C).