Key result
Adenovirus-mediated gene transfer of a Shaker potassium channel resulted in robust expression of the exogenous channel and significantly abbreviated spontaneous action potentials in rat cardiac myocytes.
Population
Rat cardiac myocytes in primary culture (in vitro) and neonatal rats (in vivo)
Comparison
Recombinant adenovirus designed to overexpress a… vs Noninfected myocytes
Design
Preclinical
Authors
Loading...
Does not support clinical use; leaves open translation of potassium channel gene therapy from rodent models to human arrhythmias.
Adenovirus-mediated gene transfer of potassium channels can successfully modify cellular action potentials in vitro and in vivo, suggesting a potential future strategy for gene therapy of arrhythmias.
Johns et al. (1995) studied Cellular excitability (preclinical model for arrhythmias). Recombinant adenovirus encoding a Drosophila Shaker potassium channel (AdShK) vs. Uninfected cells or AdLuc-infected cells was evaluated on Expression of exogenous potassium channel and modification of action potential duration. Adenovirus-mediated gene transfer of a Shaker potassium channel resulted in robust expression of the exogenous channel and significantly abbreviated spontaneous action potentials in rat cardiac myocytes.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: