Determination of Total Phenolic Content, Quercetin, and Rutin of Cosmos Caudatus Leaf Extracts and Their Contribution Toward Scavenging DPPH Radicals

C. caudatus leaves are traditionally served as a salad.The total phenolic content (TPC), rutin and quercetin levels, and inhibition against DPPH radicals were all determined in this study.This plant has a TPC of 35.891-91.321µg gallic acid equivalent/mg dried extract.Rutin and quercetin levels in this plant are approximately 17.97-18.59µg/mg and 0.73-0.79µg/mg, respectively.The extract with the highest TPC and rutin levels is 40% ethanolic extract, whereas the extract with the highest quercetin levels and DPPH radicals inhibition is 80% ethanolic extract.Furthermore, both 80% ethanolic extract and 60% ethanolic extract were classified as being similar in this investigation.Meanwhile, the good solid-to-solvent ratio employed in the extraction is 1:6 (w/v).As the outcome of this research, it was recommended that this herb be extracted using 60-80% ethanolic extract with a solid solvent ratio of 1:6.