Neuroprotective Effects of Hesperidin: In-Vitro and in-Silico Evaluation of Its Antioxidant and Enzyme Inhibitory Activities

Brain disorders are persistent medical conditions characterized by a gradual deterioration in neurological functioning.There is a worldwide increase in interest in phytomedicines for their beneficial health benefits and low adverse effects.Hesperidin (Hsp), a flavanone glycoside in citrus fruit peels, has many pharmacological characteristics.Nevertheless, there is a need for more comprehensive investigations that elucidate the underlying mechanism of action.The objective of this work is to assess the neuroprotective impact of Hsp using in-vitro tests for the inhibition of acetylcholinesterase (AChE), monoamine oxidase (MAO), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) (H2O2), followed by in-silico techniques such as molecular docking and molecular dynamics.The outcomes of the current investigation demonstrate significant inhibitory effects on AChE, MAO, DPPH, and H2O2, which may be attributed to the intended pharmacological actions of Hsp.In-silico studies showed strong interactions of Hsp with targeted proteins.Thus, Hsp has the potential to be formulated as a neuroprotective medication.