Matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry as a reliable proteomic method for characterization of Escherichia coli and Salmonella isolates

Waleed S. Shell; M.L. Sayed; Fatma Mohamed Gad Allah; Afaf Ahmed Khder; A. A. Samy; Abde Hakam M. Ali

doi:10.14202/vetworld.2017.1083-1093

September 1, 2017Veterinary WorldOpen Access

Matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry as a reliable proteomic method for characterization of Escherichia coli and Salmonella isolates

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Key Points

Evaluate matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry (MALDI-TOF MS) as a reliable proteomic diagnostic method to identify and differentiate Escherichia coli and Salmonella isolates from chickens.
Collected 110 cloacal, liver, spleen, and gall bladder samples from healthy, diseased, and freshly dead chickens showing signs such as diarrhea, pasty vent, decreased egg production, or enlarged organs across different poultry farms.
Applied bacteriological recovery and proteomic MALDI-TOF MS procedures to detect and characterize clinical bacterial isolates.
Bacterial isolates were successfully recovered from healthy and dead birds with an overall recovery rate of 92.3%.
MALDI-TOF MS demonstrated utility as an alternative routine diagnostic tool for bacterial differentiation, though further verification comparing direct colony versus extraction methods across larger sample cohorts is required.

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Implication

Diagnostic evaluation demonstrates a 92.3% recovery rate for identifying bacterial isolates in poultry samples, highlighting MALDI-TOF mass spectrometry as an alternative diagnostic tool.

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Shell et al. (2017) studied this question.

synapsesocial.com/papers/6a92ec306c5b3ee1e98efe49 https://doi.org/10.14202/vetworld.2017.1083-1093

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Matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry as a reliable proteomic method for characterization of Escherichia coli and Salmonella isolates

Key Points

No abstract yet

Implication

Discussion

Cite This Study

Matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry as a reliable proteomic method for characterization of Escherichia coli and Salmonella isolates

Key Points

No abstract yet

Implication

Discussion

Cite This Study

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