High‐Capacity Hierarchically Imprinted Polymer Beads for Protein Recognition and Capture

Leaving an imprint: Hierarchical protein imprinting starting from wide-pore silica modified with a submonolayer of adsorbed protein (IgG or HSA) leads to an inverse polymeric replica of the silica template that features highly accessible protein-complementary binding sites (see picture). The resulting poly(acrylamide) beads (PIgG or PHSA) feature high binding capacities and can be used to selectively capture the proteins HSA and IgG from blood serum.