Key result
Fluorogenic (Taqman) probes provided sensitive and reproducible detection of low-level gene expression (<10 copies/reaction), whereas SYBR Green I was only reproducible for moderate to high levels.
Population
Human and rat cytokines and growth factors
Comparison
Fluorogenic (Taqman) probes for real-time RT-PCR vs SYBR Green I detection for real-time RT-PCR
Design
Preclinical
Authors
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Fluorogenic probes merit testing in human samples; leaves open clinical translation beyond animal models.
Fluorogenic probes are superior to SYBR Green I for measuring low-level gene expression in real-time RT-PCR.
Yin et al. (2001) studied Cytokine and growth factor mRNA expression. Fluorogenic (Taqman) probes vs. SYBR Green I was evaluated on Sensitivity and reproducibility of target detection. Fluorogenic (Taqman) probes provided sensitive and reproducible detection of low-level gene expression (<10 copies/reaction), whereas SYBR Green I was only reproducible for moderate to high levels.
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