What question did this study set out to answer?

To determine how bile salts, pH, and reaction duration influence the enzymatic hydrolysis and resynthesis equilibria of triglyceride ester bonds catalyzed by pancreatic lipase.

October 1, 1964Journal of Lipid ResearchOpen Access

Influence of bile salt, pH, and time on the action of pancreatic lipase; physiological implications

View Full Paper

Ask AI

Bookmark

Authors

BBBengt BorgströmMIT Lincoln Laboratory

Discussion

Member takes

Implication

Biochemical analysis reveals that bile salts shift pancreatic lipase equilibrium toward triglyceride hydrolysis under acidic conditions, suggesting a mechanism for sustained intestinal fatty acid...

Key Points

To determine how bile salts, pH, and reaction duration influence the enzymatic hydrolysis and resynthesis equilibria of triglyceride ester bonds catalyzed by pancreatic lipase.
Measured the relative cleavage rates of 1,3- and 2-ester bonds by pancreatic lipase across varying incubation times and pH conditions.
Assessed chemical equilibrium in a two-phase oil/water system using triglycerides and the 2-monoglyceride analogue 2-octadecenyl-glycerol ether in the presence or absence of bile salts and other detergents.
Pancreatic lipase cleaved 1,3-ester bonds at 50 to 100 times the rate of 2-ester bonds, reaching a reversible steady state between ester hydrolysis and synthesis.
At pH 5.0, approximately 80% of primary hydroxyl groups remained acylated at equilibrium within the low-water interphase of the oil/water system.
Bile salts altered the reaction equilibrium under slightly acidic conditions to favor primary bond hydrolysis and monoglyceride accumulation, whereas other tested detergents inhibited lipase activity.

Cite This Study

Bengt Borgström (1964) studied this question.

synapsesocial.com/papers/6a931ac6552731dee5800ea4 https://doi.org/10.1016/s0022-2275(20)40179-8

View Full Paper

Ask AI

Bookmark

Implication

Biochemical analysis reveals that bile salts shift pancreatic lipase equilibrium toward triglyceride hydrolysis under acidic conditions, suggesting a mechanism for sustained intestinal fatty acid...

Influence of bile salt, pH, and time on the action of pancreatic lipase; physiological implications

Discussion

Member takes

Implication

Key Points

Cite This Study

Influence of bile salt, pH, and time on the action of pancreatic lipase; physiological implications

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider