Key result
A high capacity F-actin affinity matrix was constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG covalently bound to Sepharose 4B.
Population
In vitro model for constructing a high capacity F-actin affinity matrix
Design
Preclinical
Authors
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Provides stable matrix for fluorescein-tagged protein immobilization; leaves open broader actin research utility pending validation.
Anti-fluorescein IgG-Sepharose provides a stable matrix for the immobilization of proteins containing accessible, covalently bound fluorescein groups.
Luna et al. (1982) studied this question. F-actin affinity matrix was evaluated on Binding of 125I-heavy meromyosin and 125I-tropomyosin. A high capacity F-actin affinity matrix was constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG covalently bound to Sepharose 4B.
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