Key result
Heavy meromyosin (HMM) binds to F-actin with an association constant of 3 x 10(9) M-1, which is 600-fold stronger than the binding of subfragment 1 (S-1).
Population
In vitro biochemical model (heavy meromyosin and F-actin)
Comparison
Binding of heavy meromyosin (HMM) to F-actin vs Binding of subfragment 1 (S-1) to F-actin
Design
Preclinical
Authors
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May refine actomyosin binding models; leaves open in vivo cardiac implications.
Effect estimate: 600-fold stronger binding
Heavy meromyosin binds 600-fold stronger to F-actin than subfragment 1, indicating both HMM heads can bind strongly to actin.
Greene et al. (1980) studied this question. Heavy meromyosin (HMM) vs. Subfragment 1 (S-1) was evaluated on Actin-HMM association constant (600-fold stronger binding). Heavy meromyosin (HMM) binds to F-actin with an association constant of 3 x 10(9) M-1, which is 600-fold stronger than the binding of subfragment 1 (S-1).
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