The molecular mechanism of amylolytic enzymes has been investigated by differrent methods using amylose, pancreatic and Bac. subt.‐a‐amylases and amyloglucosidase. From the data obtained the following conclusions are drawn: The fitting between substrate and enzyme is induced by the C 3 hydroxyl groups of the substrate. In the course of the fitting hydrogen bonds arise between these hydroxyl groups and those of the enzyme molecule which possess the highest cohesion increment. A new theory is proposed for explaining the high catalytic effectivity of amylolytic enzymes.
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Holló et al. (1973) studied this question.
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