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High Resolution Image Download MS PowerPoint Slide Microorganisms can be genetically engineered to transform abundant waste feedstocks into value-added small molecules that would otherwise be manufactured from diminishing fossil resources. Herein, we report the first one-pot bio-upcycling of PET plastic waste into the prolific platform petrochemical and nylon precursor adipic acid in the bacterium Escherichia coli . Optimizing heterologous gene expression and enzyme activity enabled increased flux through the de novo pathway, and immobilization of whole cells in alginate hydrogels increased the stability of the rate-limiting enoate reductase BcER. The pathway enzymes were also interfaced with hydrogen gas generated by engineered E. coli DD-2 in combination with a biocompatible Pd catalyst to enable adipic acid synthesis from metabolic cis, cis -muconic acid. Together, these optimizations resulted in a one-pot conversion to adipic acid from terephthalic acid, including terephthalate samples isolated from industrial PET waste and a post-consumer plastic bottle.
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Valenzuela‐Ortega et al. (2023) studied this question.
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