What question did this study set out to answer?

To identify protein-protein interactions among CagA translocation factors and elucidate how the Cag type IV secretion system recognizes and transports CagA into host cells.

September 28, 2010Infection and ImmunityOpen Access

The Coupling Protein Cagβ and Its Interaction Partner CagZ Are Required for Type IV Secretion of the Helicobacter pylori CagA Protein

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Authors

AJAngela JurikMax Planck Institute of Psychiatry EHElisabeth HaußerLudwig-Maximilians-Universität München SKStefan KutterInstitute of Groundwater Ecology

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Implication

Molecular interaction study reveals that CagZ stabilizes coupling protein Cagβ to mediate CagA translocation in Helicobacter pylori, indicating a membrane-associated substrate-translocation complex.

Key Points

To identify protein-protein interactions among CagA translocation factors and elucidate how the Cag type IV secretion system recognizes and transports CagA into host cells.
Screened for interactions among all cag pathogenicity island genes using a yeast two-hybrid assay.
Verified direct binding and protein stability interactions among Cagβ, CagZ, and CagA via immunoprecipitation and pull-down assays.
Identified a strong physical interaction between coupling protein homologue Cagβ (HP0524) and the translocation factor CagZ (HP0526).
Demonstrated that CagZ stabilizes Cagβ and confirmed interactions between CagA and the cytoplasmic domain of Cagβ, pointing to a substrate-translocation complex at the bacterial cytoplasmic membrane.

Cite This Study

Jurik et al. (2010) studied this question.

synapsesocial.com/papers/6a9376cc368f59c7d7ff04ab https://doi.org/10.1128/iai.00796-10

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Implication

Molecular interaction study reveals that CagZ stabilizes coupling protein Cagβ to mediate CagA translocation in Helicobacter pylori, indicating a membrane-associated substrate-translocation complex.

The Coupling Protein Cagβ and Its Interaction Partner CagZ Are Required for Type IV Secretion of the Helicobacter pylori CagA Protein

Discussion

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Implication

Key Points

Cite This Study

The Coupling Protein Cagβ and Its Interaction Partner CagZ Are Required for Type IV Secretion of the Helicobacter pylori CagA Protein

Discussion

Member takes

Implication

Key Points

Cite This Study

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