Multilayer and monolayer cell cultures in a cytotoxicity assay of root canal sealers

The aim of this study was to evaluate the response of a multilayer compared with a monolayer cell culture using six root canal sealers. Both monolayer and multilayer of MU-mu-1 (Mahidol University mouse cell line 1) were cultured in separate 96-well plates. Following incubation at 37 degrees C in 5% CO2 for 4 h in the presence of each sealer, cells were stained with 0.4% Sulphorhodamine-B, viable dye staining and the absorbance at 540 nm determined and calculated as cell viability. There was no statistical difference in the percentage viability for each sealer in both the multilayer and the monolayer cell culture (P > 0.01). Apexit and AH-26 were less toxic (P < 0.01) than MU (Mahidol University) sealer, ROCANAL 3, ROCANAL 2, and Endomethasone which demonstrated the same toxicity (P > 0.01).