Hyper- and hypo-osmotic salt shocks in the presence of [32P]PO43− led to the appearance and disappearance of several 32P-labelled proteins in vivo within a few minutes. Similar to the in vivo labelling experiments, a 20.5-kDa protein was also more intensely labelled in extracts from salt-shocked cells using [γ-32P]ATP in in vitro labelling experiments. Furthermore, labelling of this and a protein of about 64.4 kDa was induced by adding high amounts of NaCl directly to extracts of control cells in kinase assays. Therefore, changes in the NaCl contents led to alterations of the activity of protein in Synechocystis sp. PCC 6803, which may be involved in the signal transduction chain sensing changes in the NaCl content of the surrounding medium.
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Hagemann et al. (1993) studied this question.