What question did this study set out to answer?

To evaluate the regulatory effects of indoleacetic acid and macromolecular synthesis inhibitors on glucanase and pectic enzyme production in pea epicotyl tissues.

May 1, 1968PLANT PHYSIOLOGYOpen Access

Indoleacetic Acid and the Synthesis of Glucanases and Pectic Enzymes

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Authors

ADAnne H. DatkoNational Institute of Mental Health GMGordon MaclachlanMcGill University

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Implication

Experimental study demonstrates auxin-induced synthesis of glucanases and pectic enzymes in decapitated pea epicotyls, highlighting hormonal regulation of cell wall modification.

Key Points

To evaluate the regulatory effects of indoleacetic acid and macromolecular synthesis inhibitors on glucanase and pectic enzyme production in pea epicotyl tissues.
Treated the apex of decapitated Pisum sativum L. var. Alaska seedlings with indoleacetic acid (IAA) alongside DNA, RNA, or protein synthesis inhibitors.
Extracted enzymic protein from epicotyl segments beneath the apex across time points up to 4 days to assay polysaccharide hydrolytic activity.
IAA treatment increased the total levels of all tested enzymes per segment except amylase.
Beta-1,4-glucanase (cellulase) activity followed a classic sigmoid induction curve per unit of protein and fresh weight.
Pectinase synthesis extended for at least 2 additional days in IAA-treated segments when cell division occurred, whereas beta-1,3-glucanase and pectinesterase increases matched total protein accumulation.

Cite This Study

Datko et al. (1968) studied this question.

synapsesocial.com/papers/6a93892d40aff459acebb5f6 https://doi.org/10.1104/pp.43.5.735

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Implication

Experimental study demonstrates auxin-induced synthesis of glucanases and pectic enzymes in decapitated pea epicotyls, highlighting hormonal regulation of cell wall modification.

Indoleacetic Acid and the Synthesis of Glucanases and Pectic Enzymes

Discussion

Member takes

Implication

Key Points

Cite This Study

Indoleacetic Acid and the Synthesis of Glucanases and Pectic Enzymes

Discussion

Member takes

Implication

Key Points

Cite This Study

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