Key result
Adiponectin stimulated apoA-1 mRNA expression in 5-day differentiated human macrophages, but did not change secreted apoA-1 or apoE protein levels.
Why the study?
Adiponectin affects energy and lipoprotein metabolism and modulates inflammation, but its role in atherogenesis remains poorly understood.
Adiponectin stimulates apoA-1 gene expression in mature human macrophages, highlighting a potential mechanism for its role in modulating atherogenesis.
Adiponectin may modulate macrophage apoA-1 in vitro; leaves open its role in atherogenesis and need for in vivo studies.
Adiponectin is an adipose tissue hormone affecting energy and lipoprotein metabolism and modulating inflammatory responses. However, the role of this adipokine in atherogenesis remains poorly understood. The aim of this study was to investigate the effect of adiponectin on the production of apolipoproteins (apo) A-1 and E by human macrophages (MP). The study was conducted on macrophage-like cells of the THP-1 cell line of two differentiation terms, 3 and 5 days (3d and 5d). Adiponectin (10 μg/mL) stimulated the expression of apoA-1 gene at the mRNA level in 5d MP, but not in 3d MP. The level of apoE mRNA in MP under the action of adiponectin was not affected. Adiponectin suppressed macrophage TNF gene expression, while it induced the expression of IL-10 gene in 5d MP. The secreted levels of apoA-1 and apoE proteins under the action of adiponectin in macrophages of both periods of differentiation remained unchanged, while the level of the surface apoA-1 protein in 5d MP was decreasing. Incubation of 5d MP with the PPARα nuclear receptor antagonist MK-886 or with the nuclear receptor LXR agonist TO-901317 resulted in cancellation of the stimulating effect of adiponectin on apoA-1 gene expression. These data indicate that adiponectin, in addition to its anti-inflammatory action, has a modulating effect on production of apoA-1 by macrophages. The latter is probably one of the mechanisms of the influence of this adipokine on atherogenesis.
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Tanyanskiy et al. (2021) studied this question. Adiponectin was evaluated on Production of apolipoproteins (apo) A-1 and E. Adiponectin stimulated apoA-1 mRNA expression in 5-day differentiated human macrophages, but did not change secreted apoA-1 or apoE protein levels.
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