What question did this study set out to answer?

Determine the direct and indirect global regulatory effects of the nutrient sensor CodY on Clostridium difficile gene transcription and virulence.

August 14, 2010Journal of BacteriologyOpen Access

Integration of Metabolism and Virulence by Clostridium difficile CodY

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Determine the direct and indirect global regulatory effects of the nutrient sensor CodY on Clostridium difficile gene transcription and virulence.
Performed DNA microarray analysis comparing global transcription profiles between wild-type Clostridium difficile and a codY null mutant.
Identified direct genomic targets using affinity purification of CodY-DNA complexes.
Verified specific CodY-DNA interactions via gel mobility shift and DNase I footprinting assays.
A codY null mutation led to >4-fold overexpression of 146 genes across 82 transcription units and underexpression of 19 genes, including derepression of toxin synthesis, amino acid biosynthesis, nutrient transport, and fermentation pathways.
Identified over 350 CodY binding regions genome-wide, with approximately 60% of CodY-repressed transcription units directly associated with physical CodY binding sites.

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Implication

Laboratory study reveals genome-wide repression of metabolic and toxin genes by CodY in Clostridium difficile, highlighting direct coupling of nutrient availability to bacterial virulence.

Cite This Study

Dineen et al. (2010) studied this question.

synapsesocial.com/papers/6a93991987f8000eec56a8b7 https://doi.org/10.1128/jb.00341-10

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Implication

Laboratory study reveals genome-wide repression of metabolic and toxin genes by CodY in Clostridium difficile, highlighting direct coupling of nutrient availability to bacterial virulence.

Integration of Metabolism and Virulence by Clostridium difficile CodY

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Discussion

Implication

Cite This Study

Integration of Metabolism and Virulence by Clostridium difficile CodY

Key Points

Discussion

Implication

Cite This Study

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