Loosening the zipper: A ligation–rearrangement reaction sequence is the key to signal amplification in chemical DNA-templated ligation reactions. The rearrangement increases the flexibility of the ligation intermediate, thereby reducing the affinity of the rearranged product for the DNA template. A fluorescence-resonant-energy-transfer technique is used for convenient and product-specific real-time detection of product signals.
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Dose et al. (2006) studied this question.
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