Key result
Addition of hemin and cofactor precursors to the culture medium yielded soluble, dimeric recombinant rat neuronal nitric oxide synthase with a specific activity of 1500 nmol.min-1.mg-1.
Population
Rat brain recombinant nitric oxide synthase expressed in a baculovirus system
Design
Preclinical
Authors
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May enable nNOS mechanistic studies; leaves open cardiovascular applications.
Absolute Event Rate: 1500% vs 50%
Optimization of culture conditions with hemin and cofactor precursors allows for the purification of highly active, soluble dimeric recombinant rat neuronal nitric oxide synthase.
Riveros‐Moreno et al. (1995) studied this question. Hemin and cofactor precursors (nicotinic acid, riboflavin, sepiapterin) was evaluated on Enzyme specific activity. Addition of hemin and cofactor precursors to the culture medium yielded soluble, dimeric recombinant rat neuronal nitric oxide synthase with a specific activity of 1500 nmol.min-1.mg-1.
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