Studies on the Inhibition of Actinomycin Biosynthesis by d-Valine

Acidic metabolites accumulate during d-valine inhibition of actinomycin formation by Streptomyces antibioticus. One of the metabolites appears to be 4-methyl-3-hydroxyanthranilic acid, a precursor of the actinomycin chromophore. The data suggest that d-valine prevents the synthesis of the antibiotic pentapeptide chain or its attachment to 4-methyl-3-hydroxyanthranilic acid. A major metabolite of d-valine is N-succinyl-d-valine.