Azo-dye was liberated from azocoll not only by proteolytic enzymes but also by albumin (serum or egg), a culture medium (papain digest of meat) or polyvinylpyrrolidone (PVP) . Pretreatment of azocoll with papain and PVP increased the reactivity of azocoll to κ-toxin of Clostridium perfringens. A rapid and reliable assay method of κ-toxin and κ-antitoxin using the pretreated azocoll is described.
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KAMEYAMA et al. (1970) studied this question.
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