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August 1, 2005Journal of Clinical MicrobiologyOpen Access

Simultaneous Detection of Hepatitis C Virus (HCV) Core Antigen and Anti-HCV Antibodies Improves the Early Detection of HCV Infection

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Authors

SLSyria LapercheÉtablissement Français du SangNMNadine Le MarrecHôpital Necker-Enfants MaladesAGA GiraultInstitut de Veille Sanitaire

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Implication

Diagnostic study demonstrates that combined antigen-antibody testing accelerates hepatitis C virus detection in blood donors and hemodialysis patients, indicating improved window-period screening.

Key Points

  • To assess whether a combined enzyme immunoassay for simultaneous detection of HCV core antigen and anti-HCV antibodies improves early detection of HCV infection compared with standard antibody, antigen, and RNA assays.
  • Tested 12 blood donor samples positive for HCV RNA and core antigen but negative for anti-HCV antibodies.
  • Evaluated 191 sequential samples (83 pre-seroconversion, 108 post-seroconversion) from 23 hemodialysis patients who seroconverted during follow-up.
  • Compared diagnostic sensitivity and detection timing of the Monolisa HCV Ag/Ab ULTRA assay against conventional anti-HCV antibody, HCV core Ag, and HCV RNA assays.
  • The Ag/Ab assay identified 6 of 12 (50%) window-period blood donor samples and 23 of 59 (39%) pre-seroconversion HCV RNA-positive hemodialysis samples, with zero positive results among 24 HCV RNA-negative samples.
  • The combination assay detected infection an average of 21.6 days earlier than the most sensitive standalone anti-HCV antibody assay.
  • Detection lagged behind HCV RNA assays by a mean delay of 30.3 days, and behind HCV core Ag screening and quantification assays by mean delays of 16.3 and 27.9 days, respectively.

Cite This Study

Laperche et al. (2005) studied this question.

synapsesocial.com/papers/6a93abb10e0cff434b8063d7https://doi.org/10.1128/jcm.43.8.3877-3883.2005
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Also Consider

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