Key result
High concentrations of MgCl2 (>10 mM) were required for the formation of ATP-resistant filaments, increased ATPase activity, and superprecipitation of unphosphorylated gizzard myosin.
Population
Gizzard unphosphorylated myosin (UM) and phosphorylated myosin (PM)
Design
Preclinical
Authors
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Phosphorylation enables myosin filament assembly at physiological MgCl2; leaves open relevance to human vascular smooth muscle regulation.
The primary effect of phosphorylation of gizzard myosin light chains is reducing the MgCl2 concentration required for the formation of ATP-resistant filaments from >10 mM to physiological levels (>2 mM).
SUZUKJ et al. (1982) studied this question. MgCl2 concentration vs. <10 mM was evaluated on Formation of ATP-resistant filaments, ATPase activity, and superprecipitation. High concentrations of MgCl2 (>10 mM) were required for the formation of ATP-resistant filaments, increased ATPase activity, and superprecipitation of unphosphorylated gizzard myosin.
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