Key result
Full-length Drosophila myosin 7a is autoregulated by its C-terminal FERM domain, which binds actin at high concentrations to unfold and activate the motor.
Population
Drosophila myosin 7a expressed in Sf9 cells
Comparison
C-terminal truncations and double mutation… vs Full-length Drosophila myosin 7a and Subfragment-1
Design
Preclinical
Authors
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Provides mechanistic insight into fly myosin regulation; leaves open whether FERM-mediated autoregulation applies to mammalian isoforms.
The C-terminal FERM domain of Drosophila myosin 7a autoregulates its activity by mediating a bent, inactive conformation at low actin concentrations.
Yang et al. (2009) studied this question. Drosophila myosin 7a-FL vs. Subfragment-1 (S1) was evaluated on MgATPase activity and actin binding. Full-length Drosophila myosin 7a is autoregulated by its C-terminal FERM domain, which binds actin at high concentrations to unfold and activate the motor.
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