Lifestyle intervention in individuals with impaired glucose regulation affects Caveolin-1 expression and DNA methylation

Figures

• 

  Figure 1.. Promoter region of Caveolin-1 (CAV1: Chromosome 7, NC_000007.14 (116524785.116561185)) showing the CpGs chosen to be analysed (numbered 1 to 12 and underlined) and the location of each assay (Assay 1 and 2, red boxes) developed to pyrosequencing those CpG targets. Results were calculated by Paired T-test and are shown as average ± SE. * p ≤ 0.025

• 

  Figure 2.. Gene expression of Caveolin-1 (CAV1) in fat tissue (a); buffy coat (b) from patients with impaired glucose regulation (IGR) at baseline and after a lifestyle change (6 months post-intervention); (c) fat tissue from patients submitted to bariatric surgery at baseline and 6 months post-surgery; (d) fat tissue from patients with T2DM history submitted to bariatric surgery at baseline and 6 months post-surgery. Results were calculated by Paired T-test and Bonferroni correction, and are shown as average ± SE. * p ≤ 0.025

• 

  Figure 3.. Sex differences in Caveolin-1 (CAV1) gene expression in fat tissue and buffy coat of patients with impaired glucose regulation (IGR) at baseline and after a lifestyle change (6 months post-intervention). Results were calculated by Paired T-test and are shown as average ± SE. * p ≤ 0.025, ** p ≤ 0.01

• 

  Figure 4.. DNA methylation levels at 12 CpG sites within the promoter region of Caveolin-1 (CAV1) in fat tissue of patients with impaired glucose regulation (IGR) at the baseline and after 6 months of lifestyle change. Results were calculated by Paired T-test and are shown as average ± SE. * p ≤ 0.025

• 

  Figure 5.. DNA methylation levels at 12 CpG sites within the promoter region of Caveolin-1 (CAV1) in buffy coat of patients with impaired glucose regulation (IGR) at the baseline and after 6 months of lifestyle change. Results were calculated by Paired T-test and are shown as average ± SE. * p ≤ 0.025

• 

  Figure 6.. LINE-1 methylation in fat tissue and buffy coat of patients with impaired glucose regulation (IGR) at the baseline and after 6 months of lifestyle change. Results were calculated by Paired T-test and are shown as average ± SE. * p ≤ 0.025

• 

  Figure 7.. Positive Pearson correlation between CpG5 of fat tissue and Caveolin-1 (CAV1) expression in buffy coat of patients with impaired glucose regulation (IGR) after the lifestyle intervention. r = 0.617, p = 0.043

• 

  Figure 8.. Negative Pearson correlation between CpG6 of buffy coat and Caveolin-1 (CAV1) expression in fat tissue of patients with impaired glucose regulation (IGR) after the lifestyle intervention. r = – 0.606, p = 0.028

• 

  Figure 9.. Negative Pearson correlation between CpG7 of buffy coat and Caveolin-1 (CAV1) expression in fat tissue of women patients with impaired glucose regulation (IGR) after the lifestyle intervention. r = – 0.959, p = 0.041

• 

  Figure 10.. (a) Positive correlation between Caveolin-1 (CAV1) gene expression and waist measurements in patients submitted to bariatric surgery (6 months post-surgery) r = 0.600; p = 0.039. (b) Positive correlation between CAV1 gene expression and waist measurements in patients with T2DM history submitted to bariatric surgery (6 months post-surgery), r = 0.931; p = 0.021