Bovine zonae were solubilized by heating to 80°C in buffered saline. On injection of female rabbits with this solution, zona‐specific antisera were produced that could be assayed on bovine eggs in vitro by immunofluorescence, coating, and inhibition of sperm‐binding. When the latter was complete, the rabbits were found to be infertile. Immunofluorescence assays showed that the rabbit anti‐bovine zona serum reacted as strongly with rabbit and rhesus monkey zonae as with homologous zonae. Strong cross‐reaction was also observed with marmoset and dog zonae, but with hamster zonae the reaction was relatively weak.
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Gwatkin et al. (1978) studied this question.
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