Why the study?
Does isoprenaline perfusion increase phospholamban phosphorylation in isolated rat hearts?
Does isoprenaline perfusion increase phospholamban phosphorylation in isolated rat hearts?
Isoprenaline induces a rapid, 3-fold increase in sarcolemmal phospholamban phosphorylation in isolated rat hearts, preceding the inotropic response.
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Does not inform clinical isoprenaline use; leaves open phospholamban timing in human myocardium.
Phosphorylation of phospholamban in cardiac sarcolemma is implicated in the increased influx of Ca2+ through the slow calcium channel induced by catecholamines. A method is described for the preparation of highly purified sarcolemmal vesicles from rat heart, and this has been used to examine the phosphorylation of phospholamban in 32Pi-perfused rat hearts. Phospholamban phosphorylation is increased 3-fold after 30 s of perfusion with 0.1 microM isoprenaline. The time course of this increase precedes the inotropic response by 5-10 s.
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Huggins et al. (1983) studied this question.
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