Key result
A novel AlphaScreen-based high throughput biochemical assay successfully determined HuR binding kinetic parameters in the nanomolar range (Kd 2.5 nM) and identified mitoxantrone as an interfering compound.
Population
Purified human HuR protein expressed in a mammalian cell-based system and ssRNA probe corresponding to TNFα…
Design
Preclinical
Authors
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No immediate clinical use; leaves open high-throughput screening of HuR modulators for future therapeutic development.
The developed AlphaScreen assay provides a robust, high-throughput method for identifying small-molecule modulators of RNA binding proteins like HuR.
D’Agostino et al. (2013) studied this question. AlphaScreen biochemical assay was evaluated on HuR binding kinetic parameters (Kd). A novel AlphaScreen-based high throughput biochemical assay successfully determined HuR binding kinetic parameters in the nanomolar range (Kd 2.5 nM) and identified mitoxantrone as an interfering compound.
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