Si-Miao-Yong-An Decoction Maintains the Cardiac Function and Protects Cardiomyocytes from Myocardial Ischemia and Reperfusion Injury

Figures

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  Figure 1. Timeline graph of animal experiments.

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  Figure 2. The coronal view diagram of CMR and the effect of SMYAD on cardiac functions of I/R mice model (n = 3). LA: left atrial; LV: left ventricular; RV: right ventricular. LV-EDA: left ventricular end-diastolic volume; LV-ESV: left ventricular end-systolic volume; EF: ejection fraction. ∗p < 0.05 and ∗∗p < 0.01 versus control group; #p < 0.05 and ##p < 0.01 versus I/R group.

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  Figure 3. The effect of SMYAD on pathological changes of I/R mice model in the heart tissue (n = 3). (a, b) Representative images of H&E staining and Masson staining in the whole heart, respectively. Scale bar is 1 mm. (c) The results of the quantitative analysis of Masson staining using ImageJ.

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  Figure 4. The effect of SMYAD on the expressions of collagen I, MMP9, and TNFα protein in the heart tissue of I/R mice model (n = 3). (a, b) Protein levels of collagen I, MMP9, and TNFα in each group by western blot. ∗p < 0.05 and ∗∗p < 0.01 versus control group; #p < 0.05 and ##p < 0.01 versus I/R group.

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  Figure 5. The protective effect of different doses of SMYAD (0, 50, 75, 100, 125, and 150 μg/mL) on the viability of cultured H9C2 cardiomyocytes under the normal and H/R conditions (n = 6), as determined by the MTT assay kit. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus H/R group.

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  Figure 6. The effect of SMYAD on autophagy of myocardial cells injured by H/R model. (a, b) Protein levels of p-mTOR, mTOR, and LC3B-I/II in each group by western blot (n = 3). ∗∗p < 0.01 versus control group; #p < 0.05 and ##p < 0.01 versus I/R group.

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  Figure 7. The effect of SMYAD on pyroptosis of myocardial cells injured by H/R model. (a, b) Protein levels of NLRP3, procaspase 1, and cleaved-caspase 1 in each group by western blot (n = 3). (c) Expression level of IL-1β by ELISA in each group. ∗∗p < 0.01 versus control group; #p < 0.05 versus I/R group.