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January 27, 2011Journal of Clinical MicrobiologyOpen Access

Simultaneous Detection of Influenza A, Influenza B, and Respiratory Syncytial Viruses and Subtyping of Influenza A H3N2 Virus and H1N1 (2009) Virus by Multiplex Real-Time PCR

Yu Chen Dawei Cui Shufa Zheng Shigui Yang Dagan Yang Jian‐Gao Fan Jie Zhang Bin Lou Xuefen Li Xiaoling Zhuge Bo Ye Baode Chen Weilin Mao Yajun Tan Gen-Yun Xu Zhenjin Chen Nan Chen Lanjuan Li

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Abstract

A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza A virus subtypes, including novel H1N1 (2009) and seasonal H3N2 virus, influenza B virus, and respiratory syncytial virus (RSV) in a single test tube, with detection sensitivity and specificity of 99% and 100%, respectively, for the four pathogens.

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Chen et al. (2011) studied this question.

synapsesocial.com/papers/6a93cb112ab0e6a83607e104 https://doi.org/10.1128/jcm.02184-10

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Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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