What question did this study set out to answer?

To develop a sensitive and specific radioimmunoassay capable of accurately measuring bioactive plasma cholecystokinin without interference from homologous gastrin peptides.

May 1, 1998Clinical Chemistry

Accurate measurement of cholecystokinin in plasma

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To develop a sensitive and specific radioimmunoassay capable of accurately measuring bioactive plasma cholecystokinin without interference from homologous gastrin peptides.
Raised antisera against a sulfated CCK-12 analog across rabbits, guinea pigs, and mice, screening titers from 200 to 4,000,000 against homologous peptides and plasma extracts.
Evaluated plasma CCK concentrations before and after ingestion of a mixed meal in healthy humans (n = 26) and characterized circulating forms via plasma chromatography.
Rabbit antiserum 92128 exhibited high avidity (effective affinity constant >= 10^12 mol^-1), bound bioactive CCK forms with equimolar potency, and displayed no cross-reactivity with gastrin.
Plasma CCK concentrations in healthy subjects increased from 1.13 +/- 0.10 pmol/L before the meal to 4.92 +/- 0.34 pmol/L after the meal (mean +/- SE, n = 26).
Chromatographic fractionation of human plasma identified a predominance of CCK-33 and CCK-22, moderate concentrations of CCK-8, and trace amounts of CCK-58.

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Implication

Experimental study demonstrates sensitive and specific measurement of plasma cholecystokinin in healthy humans, indicating reliable discrimination from cross-reactive gastrin.

Cite This Study

Jens F. Rehfeld (1998) studied this question.

synapsesocial.com/papers/6a93d79ca0db6e712b871f05 https://doi.org/10.1093/clinchem/44.5.991

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Implication

Experimental study demonstrates sensitive and specific measurement of plasma cholecystokinin in healthy humans, indicating reliable discrimination from cross-reactive gastrin.

Accurate measurement of cholecystokinin in plasma

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Discussion

Implication

Cite This Study

Accurate measurement of cholecystokinin in plasma

Key Points

Discussion

Implication

Cite This Study

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