What question did this study set out to answer?

To identify and characterize a novel conserved protein sequence motif shared among proteins implicated in membrane trafficking and structural regulation across diverse eukaryotes.

January 1, 1999Protein ScienceOpen Access

Identification of a novel domain shared by putative components of the endocytic and cytoskeletal machinery

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Authors

BKBrian K. KayTango Therapeutics (United States)MYMontarop YamabhaiBiotechnology Research Center BWBeverly WendlandJohns Hopkins University

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Implication

Comparative sequence analysis uncovers a conserved 140-amino-acid ENTH domain across eukaryotic proteins, suggesting roles in endocytic trafficking and cytoskeletal regulation.

Key Points

To identify and characterize a novel conserved protein sequence motif shared among proteins implicated in membrane trafficking and structural regulation across diverse eukaryotes.
Analyzed sequence conservation and domain architecture in 12 homologous proteins across budding yeast, fission yeast, nematodes, rodents, frogs, plants, and humans.
Mapped conserved residues and relative positions near the N-termini across species.
Identified a ~140 amino acid sequence, termed the epsin N-terminal homology (ENTH) domain, typically located within 20 residues of the N-terminus.
Sequence identity among the 12 proteins ranged from 42% to 93%, harboring 16 invariant amino acid residues within a defined consensus pattern.
Homology analysis linked the ENTH domain to proteins functioning in endocytosis and cytoskeletal organization, implicating it in ligand binding or enzymatic function.

Cite This Study

Kay et al. (1999) studied this question.

synapsesocial.com/papers/6a93dbe2219df916ad43dc9b https://doi.org/10.1110/ps.8.2.435

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Implication

Comparative sequence analysis uncovers a conserved 140-amino-acid ENTH domain across eukaryotic proteins, suggesting roles in endocytic trafficking and cytoskeletal regulation.

Identification of a novel domain shared by putative components of the endocytic and cytoskeletal machinery

Discussion

Member takes

Implication

Key Points

Cite This Study

Identification of a novel domain shared by putative components of the endocytic and cytoskeletal machinery

Discussion

Member takes

Implication

Key Points

Cite This Study

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