What question did this study set out to answer?

To evaluate the apoptotic effects and underlying molecular mechanisms of fucoidan in human colon cancer cell lines.

August 22, 2010BMC GastroenterologyOpen Access

Fucoidan present in brown algae induces apoptosis of human colon cancer cells

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Authors

EKEun Ji KimNorthwell Health SPSo Young ParkCatholic University of Daegu JLJae‐Yong LeeHallym University

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Implication

In vitro study demonstrates that fucoidan triggers apoptosis in human colon cancer cells, suggesting its potential utility in colon cancer prevention.

Key Points

To evaluate the apoptotic effects and underlying molecular mechanisms of fucoidan in human colon cancer cell lines.
Cultured HT-29 and HCT116 human colon cancer cells with fucoidan at concentrations ranging from 0 to 20 µg/mL.
Assayed apoptosis, cell viability, and mitochondrial membrane permeability using Hoechst staining, Annexin V flow cytometry, and JC-1 staining.
Measured levels of apoptosis-regulating proteins and mitochondrial release factors by Western blot analysis, with and without caspase inhibitors.
Fucoidan reduced viable cell numbers and induced apoptosis in HT-29 and HCT116 cells in a dose-dependent manner.
Treatment increased cleaved caspases-8, -9, -7, and -3, cleaved PARP, Bak, truncated Bid, TRAIL, Fas, and DR5, while downregulating XIAP, survivin, and Mcl-1.
Fucoidan elevated mitochondrial membrane permeability and the release of cytochrome c and Smac/Diablo, with apoptosis attenuated by caspase-8 and caspase-9 inhibitors.

Cite This Study

Kim et al. (2010) studied this question.

synapsesocial.com/papers/6a93e1657fb89795fbfec7fb https://doi.org/10.1186/1471-230x-10-96

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Implication

In vitro study demonstrates that fucoidan triggers apoptosis in human colon cancer cells, suggesting its potential utility in colon cancer prevention.

Fucoidan present in brown algae induces apoptosis of human colon cancer cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Fucoidan present in brown algae induces apoptosis of human colon cancer cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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