Differential Production of Interferon and Refractoriness Inducing Principle in L Cells

The extracellular, acid-soluble cell products (EASCP) from Newcastle disease virus-infected L(929) cells contain both interferon, defined as antiviral activity, and refractoriness inducing principle, defined as an activity that inhibits interferon production. L cells pretreated with EASCP and then infected with Newcastle disease virus give rise to EASCP with decreased amounts of interferon but an increased ratio of refractoriness inducing principle activity to interferon activity in a dose related manner. The antiviral activity of an EASCP preparation is not dependent upon its refractoriness inducing principle level, but is entirely dependent on its interferon content. Our results provide additional evidence that interferon and refractoriness inducing principle are different biological entities and not polymorphic functions of the interferon molecule.