Quantitative Analysis of Cannabidiol and Δ9-Tetrahydrocannabinol Contents in Different Tissues of Four Cannabis Cultivars using Gas Chromatography-Mass Spectrometry

In this study, we analyzed cannabidiol (CBD) and 9-tetrahydrocannabinol (THC) contents in different tissues of four cannabis cultivars, Cheongsam, Cherry Blossom, Hot Blonde, and Queen Dream, using gas chromatography-mass spectrometry (GC-MS). Standard calibration curves for CBD and THC were generated by analyzing standard CBD and THC solutions via GC-MS, respectively. GC-MS analyses of different tissues of the Cheongsam cultivar revealed different levels of CBD and THC depending on the tissues tested. Female flowers accumulated the highest CBD and THC levels at 0.0520.02 and 0.020.005%, respectively, in the Cheongsam cultivar. Similarly, female flowers of the Cherry Blossom, Hot Blonde, and Queen Dream cultivars accumulated the highest amounts of CBD (2.45% to 3.54%) and THC (0.15% to 0.18%) compared to other tissues, such as the leaves and stems. These results suggest that female flowers of cannabis plants show higher potency levels for CBD and THC production compared to other tissues.