What does this research mean for the field?

A newly established protocol using propidium iodide staining and flow cytometry of antheridia enables reliable nuclear visualization and genome size estimation in Charophyceae, revealing extensive nuclear diversity across cell types. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To develop a reliable nuclear visualization protocol for Charophyceae, characterize cell- and species-specific nuclear organization, and identify suitable cell types for flow cytometric genome size estimation.

August 30, 2026PlantaOpen Access

Nuclear diversity in Charophyceae revealed by fluorescence staining and its implications for genome size estimation

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Authors

JBJulien Böhm JPJulia Peters KHKlaus Herburger

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Overview

Cytological analysis reveals high nuclear diversity across tissues in Charophyceae, indicating antheridia are optimal for reliable flow cytometric genome size estimation.

Key Points

To develop a reliable nuclear visualization protocol for Charophyceae, characterize cell- and species-specific nuclear organization, and identify suitable cell types for flow cytometric genome size estimation.
Screened three DNA-intercalating fluorochromes and employed fluorescence microscopy alongside transmission electron microscopy to verify nuclear morphology across Characeae species.
Evaluated nuclear patterns across vegetative and reproductive tissues in Chara hispida, Chara tomentosa, and Sphaerochara canadensis.
Conducted flow cytometry on whole-thallus samples and isolated antheridia to determine genome size.
Propidium iodide provided the most reliable nuclear labeling, revealing mononucleated apical, coronula, and spermatogenous cells alongside predominant multinucleation in cortical, spine, stipulode, and rhizoid tissues.
Chara hispida exhibited an axial gradient with larger, heterogeneous nuclei in apical internodes and smaller, uniform nuclei in basal internodes, consistent with potential endopolyploidy.
Whole-thallus flow cytometry failed to yield a defined nuclear peak, whereas isolated Chara tomentosa antheridia generated a distinct peak yielding an estimated 1C genome size of 5.32 pg.

Cite This Study

Böhm et al. (2026) studied this question.

synapsesocial.com/papers/6a93f0c06c1a8fb52e79d2db https://doi.org/10.1007/s00425-026-05085-w

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August 30, 2026PlantaOpen Access

Nuclear diversity in Charophyceae revealed by fluorescence staining and its implications for genome size estimation

View Full Paper

Ask AI

Bookmark

Authors

JBJulien Böhm JPJulia Peters KHKlaus Herburger

Discussion

Member takes

Overview

Cytological analysis reveals high nuclear diversity across tissues in Charophyceae, indicating antheridia are optimal for reliable flow cytometric genome size estimation.

Key Points

To develop a reliable nuclear visualization protocol for Charophyceae, characterize cell- and species-specific nuclear organization, and identify suitable cell types for flow cytometric genome size estimation.
Screened three DNA-intercalating fluorochromes and employed fluorescence microscopy alongside transmission electron microscopy to verify nuclear morphology across Characeae species.
Evaluated nuclear patterns across vegetative and reproductive tissues in Chara hispida, Chara tomentosa, and Sphaerochara canadensis.
Conducted flow cytometry on whole-thallus samples and isolated antheridia to determine genome size.
Propidium iodide provided the most reliable nuclear labeling, revealing mononucleated apical, coronula, and spermatogenous cells alongside predominant multinucleation in cortical, spine, stipulode, and rhizoid tissues.
Chara hispida exhibited an axial gradient with larger, heterogeneous nuclei in apical internodes and smaller, uniform nuclei in basal internodes, consistent with potential endopolyploidy.
Whole-thallus flow cytometry failed to yield a defined nuclear peak, whereas isolated Chara tomentosa antheridia generated a distinct peak yielding an estimated 1C genome size of 5.32 pg.

Cite This Study

Böhm et al. (2026) studied this question.

synapsesocial.com/papers/6a93f0c06c1a8fb52e79d2db https://doi.org/10.1007/s00425-026-05085-w

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