Abstract 9‐d‐Hydroxy‐cis‐12‐octadecenoic acid has been isolated from 3 seed oils of the family Apocynaceae:Holarrhena antidysenterica (73%),Nerium oleander (11%) andNerium indicum (8%). The known occurrence of this acid was previously limited to the genusStrophanthus (9–15%). A mixture of unusual tetra‐acid glycerides was isolated fromN. oleander oil by thin layer chromatography. Pancreatic lipase hydrolysis of the glyceride mixture showed that 9‐d‐acetoxy‐cis‐12‐octadecenoic acid is esterified exclusively at an α‐glycerol position and that normal fatty acids occupy the remaining 2 glycerol positions. A portion of the hydroxy acid inN. indicum oil was also acetylated, however, no acetate was found in eitherH. antidysenterica orStrophanthus hispidus oils.
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Powell et al. (1969) studied this question.
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