Transcriptome sequencing analysis of echovirus 30 infection reveals its potential pathogenesis

Figures

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  FIGURE 1. Expression profile for echovirus 30 infection in human glioma (U251) cells. (A) Change in E30 viral titer in U251 cells, (B) CPE appeared at 48 h after infection of U251 cells with E30, (C,D) sequencing and information analysis flow chart, and (E) heat map analysis of E30-infected and control group (each group, n = 3).

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  FIGURE 2. Functional classification of differentially expressed genes (DEGs) in E30-infected human glioma (U251) cells. (A) Gene Ontology (GO) enrichment analysis of DEGs. (B) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of DEGs. (C) Visualization of DEGs enriched in E30-infected U251 cells. (D,E) Molecular interaction between DEGs and STRING randomly draws colors based on the scores of interacting genes and is labeled with gene names.

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  FIGURE 3. E30 infects ICR suckling mice. (A) Body weight, clinical score, and survival in ICR suckling mice infected with E30. (B) Clinical symptoms of E30 infection in ICR suckling mice. (C) Virus tissues in brain tissues 5 days after infection.

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  FIGURE 4. Functional classification of differentially expressed genes (DEGs) in E30-infected suckling mice brain tissue. (A,B) Principal component analysis (PCA) and heat map analysis of E30-infected brain tissue and control infection group (each group, n = 3), (C) enriched pathways among DEGs in biological processes, (D) cellular components, and (E) molecular function terms. High P-values are in blue and low P-values are in red. The size of the spots indicates the number of genes.

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  FIGURE 5. Visualization of differentially expressed genes (DEGs) in echovirus 30-infected suckling mice brain tissue. (A,C) GO bar plot analysis of DEGs enriched in GO terms in the brain tissue and (B,D) visualization of DEGs enriched in the brain tissue using KEGG. (E) The log2 fold change of differentially expressed genes in the RNA-Seq data and validation of differentially expressed genes via RT-PCR assays.