Key result
Eleven newly developed fluorescence resonance energy transfer (FRET) assays successfully detected 15 polymorphisms in endothelin genes, offering a faster alternative to previous methods.
The development of these FRET assays provides a faster, more cost-effective method for genotyping endothelin system polymorphisms, which may be useful for predicting vascular disease susceptibility.
Endothelins (EDNs) are peptides, produced by various tissues, with potent vasoactive and mitogenic properties. Endothelin actions are mediated via specific G protein-coupled receptors of two subtypes, endothelin-receptor-A (EDNRA) and endothelin-receptor-B (EDNRB). Some polymorphisms of the EDN1, EDN2 and EDNRA genes may influence susceptibility to vascular diseases. Thus, genotyping for polymorphisms of these genes may represent a tool for predicting individual susceptibility to vascular diseases. Here, we report 11 fluorescence resonance energy transfer (FRET) assays for the detection of 15 polymorphisms, because the assays used in previous studies (allele-specific PCR and restriction fragment-length polymorphism assays) for some of these polymorphisms are laborious and time-consuming. The newly developed assays are fast and work without expensive ready-to-use mixtures.
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Arjomand-Nahad et al. (2006) studied this question. 11 fluorescence resonance energy transfer (FRET) assays vs. allele-specific PCR and restriction fragment-length polymorphism assays was evaluated on Detection of 15 polymorphisms. Eleven newly developed fluorescence resonance energy transfer (FRET) assays successfully detected 15 polymorphisms in endothelin genes, offering a faster alternative to previous methods.
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